The extensive use of atrazine to control weeds in agricultural areas has contaminated atrazine in surface water and groundwater. Atrazine contamination in water resources causes human health concerns. Thus, this study investigated the possible use of aquatic plants for removing atrazine from contaminated water. The experiment was performed under plant nursery conditions and divided into two parts: (1) the atrazine-tolerant plants were screened, and (2) the most atrazine-tolerant plant was used for atrazine phytoremediation stimulated by plant growth regulators. The results showed that atrazine was toxic to all aquatic plants, as the dry weight of the plants was significantly decreased when exposed to 20 mg/L of atrazine (P<0.05). Based on five aquatic plants grown under 2.5–20 mg/L atrazine-contaminated water, Azolla microphylla Kaulf. was the most tolerant aquatic plant and was more suitable for use in atrazine phytoremediation than the other aquatic plants (Ceratophyllum demersum L., Eichhornia crassipes (Mart.) Solms, Hydrilla verticillata (L. f.) Royle, and Salvinia cucullata Roxb. ex Bory). The total chlorophyll, carotenoid, and proline contents in the biomass of A. microphylla cultured in 2.5–20 mg/L of atrazine did not significantly differ between the atrazine concentrations (P>0.05). Meanwhile, the proline contents in the other four aquatic plants increased with increasing atrazine concentrations, and the chlorophyll content significantly decreased with an increase in the atrazine concentration. However, A. microphylla could not remove atrazine from contaminated water, and the application of plant growth regulators (6-benzyladenine, gibberellic acid, indole-3-butyric acid, and salicylic acid) did not improve the atrazine removal from water. Atrazine in the water was around 21–26 mg/L on day five of A. microphylla cultivation compared to the initial concentration (25 mg/L). Using a plant growth regulator was ineffective for stimulating growth and atrazine removal by A. microphylla. Future research should explore other potential mechanisms for enhancing atrazine removal by A. microphylla.

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