This review paper aims to provide a comprehensive overview of hormonal profiling in post-partum dairy buffaloes, elucidating its significance in reproductive management, milk production, and overall herd health. We discuss the hormonal changes occurring during the post-partum period, factors influencing these changes, current methods of hormonal assessment, and their applications in reproductive and productive efficiency. Furthermore, we address challenges and gaps in existing research and propose future directions for advancing hormonal profiling techniques in dairy buffalo management.