The quality of fruits, including durian, can be significantly affected by the age of the tree. The impact of tree age on the physicochemical, antioxidant, and mineral compositions of Musang King durian (MKD) fruit was investigated using physicochemical and biochemical approaches. The results revealed that firmness, pH, reducing sugar, polyphenol, and antioxidant activity were affected by tree age. Durian fruits from old trees exhibited higher firmness and reduced sugar compared to young and middle-aged trees. Fruits from old and middle-aged trees had higher polyphenol and antioxidant activity compared to young trees durian fruits. Calcium and nitrogen were significantly higher in durian fruits from young trees, while potassium and zinc were higher in fruits from old trees. The finding demonstrated that tree age impacts the physicochemical, antioxidant content, and mineral composition of MKD fruits, and these changes may respond to the sensory perception of MKD fruits.